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Enzymes for Molecular Biology
DNA Polymerases See also our special offer for Diamond Mix.
Each polymerase has different characteristics, so it is crucial to choose the enzyme that suits your individual experiment. Bioline polymerases are available alone but are also supplied as convenient ready-to-use 2x Reaction Mixes containing the polymerase of choice plus dNTPs, MgCl2 and further additives to achieve optimal reaction conditions.
| Polymerase |
Suitable
Template Lenght |
Heat Activated |
Sensitivity / Low
Copy Templates |
Elongation
required (bases/sec) |
Specificity |
Problem
Templates |
Fidelity |
GC-rich
Templates |
Performance |
3'End
Modification |
Yield |
| ACCUZYME |
Up to 5Kb |
No |
* |
20 - 40 |
* |
* |
*** |
* |
** |
Blunt |
** |
ACCUZYME
Red |
Up to 5Kb |
No |
* |
20 - 40 |
* |
* |
*** |
* |
** |
Blunt |
** |
| ACCUZYME Mix |
Up to 5Kb |
No |
* |
20 - 40 |
* |
* |
*** |
* |
** |
Blunt |
** |
| AccuSure |
Up to 5Kb |
Yes |
* |
20 - 40 |
** |
* |
*** |
* |
** |
Blunt |
** |
| AccuSure Mix |
Up to 5Kb |
Yes |
* |
20 - 40 |
** |
* |
*** |
* |
** |
Blunt |
** |
| SAHARA |
Up to 5Kb |
Yes |
*** |
|
*** |
*** |
** |
* |
** |
Mix (3'-dA + Blunt) |
*** |
| IMMOLASE |
Up to 5Kb |
Yes |
** |
30 - 70 |
*** |
* |
- |
* |
** |
3'-dA |
*** |
ImmoMix /
ImmoMix Red |
Up to 5Kb |
Yes |
** |
30 - 70 |
*** |
* |
- |
* |
** |
3'-dA |
*** |
|
BIO-X-ACT
Short |
Up to 5Kb |
No |
** |
40 - 60 |
** |
*** |
** |
* |
** |
Mix (3'-dA + Blunt) |
*** |
|
BIO-X-ACT
Short Mix |
Up to 5Kb |
No |
** |
40 - 60 |
** |
*** |
** |
* |
** |
Mix (3'-dA + Blunt) |
*** |
|
BIO-X-ACT
Long |
3 - 20Kb |
No |
** |
40 - 60 |
** |
*** |
** |
** |
** |
Mix (3'-dA + Blunt) |
** |
|
BIO-X-ACT
Long Mix |
3 - 20Kb |
No |
** |
40 - 60 |
** |
*** |
** |
** |
** |
Mix (3'-dA + Blunt) |
** |
|
Diamond |
Up to 5Kb |
No |
* |
20 - 30 |
*** |
*** |
* |
*** |
* |
3'-dA |
* |
|
BIOTAQ /
BIOTAQ Red |
Up to 5Kb |
No |
** |
30 - 70 |
* |
- |
- |
* |
*** |
3'-dA |
*** |
|
BioMix /
BioMix Red |
Up to 5Kb |
No |
** |
30 - 70 |
* |
- |
- |
* |
*** |
3'-dA |
*** |
|
Mango Taq |
Up to 5Kb |
No |
** |
30 - 70 |
* |
- |
- |
* |
** |
3'-dA |
** |
| MangoMix |
Up to 5Kb |
No |
** |
30 - 70 |
* |
- |
- |
* |
** |
3'-dA |
** |
For more information, please download the Bioline PCR Guide or contact GC biotech to request a Bioline catalog (info@gcbiotech.com).
Enzymes
Klenow Fragment:
Features
* Produces blunt ends
* Lacks exonuclease activity
* Sequencing grade
Applications
* Second strand cDNA synthesis
* Filling-in 5' Ends
* Radio labeling and dideoxy sequencing
Uracil DNA Glycosylase (UDG):
Features
* Recombination Source
* DNase/RNase-free
* Supplied with 10x Reaction Buffer
Application
* Removal of Uracil from Uracil-containing DNA
For more information contact GC biotech to request a Bioline catalog (info@gcbiotech.com). |
