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 Fully Automated genomic DNA isolation from large volume of blood

Fully Automated genomic DNA isolation from large volume of blood

 

Introduction

A very reliable, fully automated, highly reproducible DNA isolation station, to isolate genomic DNA from large volume blood (up to 10 ml). The set up of the platform consists of a Xperimate liquidhandler and one or two Chemagic Separation Module 1’ns of Chemagen. The Xperimate is equipped with 5 ml disposable. The Chemagen MSM1 uses a dispenser to add the reagents to the blood samples and runs the Chemagic DNA Blood Kit.

 

 

 Features

·         Fully automated, no manual interactions

·         5 ml carbon tips (liquid level & clot detection)

·         Two configurations; One or two Chemagen MSM1’ns integrated on one Xperimate Liquid handler

·         Barcode tracking

·         From 1 to 10 ml blood

·         Flexispan disposable tip probes

·         LIMS connection

·         No need to rehydrate your DNA, immediately available

·         Isolates up to 192 samples in 8 hours

 

 

Gripper for tubes and racks                           5 ml tips with liquid level detection

 

Results

Volume Blood (ml)

Average yield [ug]

Yield *[ug/mL]

2

56

28

5

132

25

7

173

24

Table: Yield gDNA from whole blood

*) Concentration measurement on Nanodrop

 

 

 

Top view configuration Xperimate with two Chemagen MSM1’ns

 

 

 

This application is a co-development of Synchron Lab Automation, specialists in robotics & engineering, and GC biotech, specialists in Life Sciences & robotics.